Fruit and vegetables play a crucial role in ensuring food and nutrition security, and developing more sustainable value chains in agriculture and the agri-food sector. To support a greater supply of fruit and vegetables, small farmers’ production is fundamental and needs to be integrated into stable value chains to maintain market, logistics and quality conditions. This article develops a theoretical framework based on the conditions, strategies and performances of supply chain systems, combined with the elicitation of expert opinion, to identify key variables for the specific analysis of fruit and vegetable supply chains. Empirical data was retrieved from eight supply chains in five Mediterranean countries to identify the most relevant issues related to their conditions, strategies and performances. Three different types of supply chains were included: 1) Short food supply chains, 2) Green public procurement, and 3) Exportoriented supply chains. This research made it possible to identify key indicators for the analysis of fruit and vegetable supply chain system dynamics. The variables identified in this study may contribute to prospective research for the assessment of fruit and vegetable supply chain sustainability and to the development of policies that encourage the adoption of environmentally-friendly and socially-responsible practices, thus contributing to the long-term sustainability of Mediterranean fruit and vegetables supply chains.